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一、沙門氏菌微生物檢測板產品介紹
1、原理及適用范圍
沙(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)菌(jun)屬腸道細(xi)菌(jun)科(ke)，包括那些(xie)引起食(shi)物(wu)中(zhong)毒、導致腸胃炎、傷(shang)寒和(he)(he)(he)副傷(shang)寒的(de)(de)細(xi)菌(jun)。能引起食(shi)物(wu)傳(chuan)播(bo)性疾病，近年來，已經(jing)成為(wei)常(chang)見的(de)(de)食(shi)物(wu)中(zhong)毒原因。腸炎沙(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)菌(jun)感(gan)染通常(chang)源于奶制品(pin)(pin)(pin)、禽(qin)產(chan)品(pin)(pin)(pin)和(he)(he)(he)肉產(chan)品(pin)(pin)(pin)；雞肉和(he)(he)(he)雞蛋尤其是高風險食(shi)品(pin)(pin)(pin)。沙(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)菌(jun)感(gan)染的(de)(de)癥狀通常(chang)是腸胃出(chu)現問題，包括惡心(xin)、腹部絞痛、嘔吐和(he)(he)(he)腹瀉，一般*多持續7天(tian)。在免疫力低下的(de)(de)人(ren)群中(zhong)，如果不及(ji)時服用抗生(sheng)(sheng)素(su)類藥品(pin)(pin)(pin)，沙(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)菌(jun)感(gan)染將導致生(sheng)(sheng)命危險。沙(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)菌(jun)檢(jian)(jian)測板含有(you)選擇性培養(yang)基、沙(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)菌(jun)*辛酯酶的(de)(de)顯(xian)色指(zhi)示劑和(he)(he)(he)高分(fen)子(zi)吸(xi)水(shui)凝(ning)膠，運(yun)用微生(sheng)(sheng)物(wu)檢(jian)(jian)測板專(zhuan)有(you)技術，做成一次性快(kuai)速檢(jian)(jian)驗(yan)產(chan)品(pin)(pin)(pin)，一步培養(yang)15～24 h就可(ke)確認是否(fou)帶有(you)沙(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)菌(jun)，非常(chang)適(shi)合各級檢(jian)(jian)驗(yan)部門(men)(men)和(he)(he)(he)食(shi)品(pin)(pin)(pin)企業使(shi)用。執行標(biao)準：中(zhong)華人(ren)民共和(he)(he)(he)國國家標(biao)準《食(shi)品(pin)(pin)(pin)安全(quan)國家標(biao)準 微生(sheng)(sheng)物(wu)學檢(jian)(jian)驗(yan) 沙(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)菌(jun)檢(jian)(jian)驗(yan)》（GB 4789 .4）。
2、沙門氏菌微生物檢測板操作方法
2.1 樣(yang)(yang)品處理(li)：取樣(yang)(yang)品 25 mL（g）放入含(han)有225 mL滅菌磷酸緩沖液(ye)（或生理(li)鹽(yan)水）的取樣(yang)(yang)罐或均質(zhi)袋(dai)內，制成(cheng)1:10的樣(yang)(yang)品勻液(ye)。根據樣(yang)(yang)品污染程度及檢驗需要，可進(jin)一步制成(cheng)10倍(bei)遞增的樣(yang)(yang)品稀(xi)釋液(ye)。
2.2 接種(zhong)：將沙門氏菌檢測板置(zhi)(zhi)于平坦實驗臺面，揭(jie)開上(shang)層膜，用無(wu)菌吸(xi)管(guan)吸(xi)取1mL樣(yang)品(pin)(pin)勻液滴加到檢測板內(nei)，迅速貼好上(shang)層膜并水(shui)平晃動檢測板，待樣(yang)品(pin)(pin)勻液均勻吸(xi)附在濾(lv)紙(zhi)上(shang)，靜置(zhi)(zhi)10 s左右，待樣(yang)品(pin)(pin)勻液被吸(xi)附在濾(lv)紙(zhi)上(shang)。每個稀釋度(du)接種(zhong)兩(liang)片。
2.3 培(pei)養(yang)：將檢(jian)測板(ban)疊在(zai)一起(qi)，倒置放于培(pei)養(yang)箱內。36℃±1℃，培(pei)養(yang)15～24 h。
3、結果判讀
對檢測(ce)板(ban)進(jin)行(xing)觀察，呈(cheng)(cheng)紫紅色的菌(jun)(jun)落(luo)為沙(sha)門氏菌(jun)(jun)；呈(cheng)(cheng)藍色的菌(jun)(jun)落(luo)為其(qi)(qi)他菌(jun)(jun)群。出現陽性菌(jun)(jun)落(luo)的樣(yang)本，*好(hao)用其(qi)(qi)他更為可靠的方(fang)法(fa)進(jin)行(xing)驗(yan)證，或者(zhe)至(zhi)少要(yao)再取樣(yang)重復檢驗(yan)一(yi)次(ci)。
4、附加說明 
4.1 有關驗證試驗表明，接種純菌(jun)種（包括硫化(hua)氫(qing)陰性菌(jun)株）可以產生(sheng)典(dian)型的紫(zi)紅色(se)菌(jun)斑，其他(ta)腸桿菌(jun)呈藍色(se)，葡萄球菌(jun)不生(sheng)長。
4.2 注意使用(yong)過的檢測板上(shang)帶(dai)有活菌，需(xu)及時按照生物(wu)安全(quan)廢棄物(wu)處理(li)原則進行處理(li)。